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Home Nucleic Acid Purification RNA Direct-zol RNA Microprep (Product Supplied w/ 200 ml TRI Reagent) R2063
Direct-zol™ RNA MicroPrep (200 Preps) w/ Zymo-Spin™ IC Columns (Capped) (Product Supplied w/ 200 ml TRI Reagent™) [Includes R2052 x 1, R2050-1-200 x 1 - packaged separately]

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Direct-zol RNA Microprep (Product Supplied w/ 200 ml TRI Reagent)

Isolate total RNA and small/miRNA from TRIzol® without phase separation

Cat # Name Size
R2063
Direct-zol™ RNA MicroPrep (200 Preps) w/ Zymo-Spin™ IC Columns (Capped) (Product Supplied w/ 200 ml TRI Reagent™) [Includes R2052 x 1, R2050-1-200 x 1 - packaged separately]
200 preps

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DESCRIPTION

A streamlined method for the purification of up to 10 µg (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol™ method assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin™ Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencing etc.). The entire procedure typically takes only 7 minutes.

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