Biolinkk

25 mM MgCl2

25 mM MgCl2
For research use only.

Some applications of this product  may require a license which is not provided by the purchase of this product.

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Description
Stock solution for PCR assay optimization.
25 mM MgCl2 is used for adjusting Mg2+ concentration in PCR assay.

* Additive for (q)PCR
* Additive for RT-(q)PCR

MgCl2 solution is used for adjusting Mg2+ concentration in PCR assay. It can be used in combination with PCR buffers that come with FIREPol® and HOT FIREPol® which are all MgCl2 free). In most applications, a final concentration of 1,5 mM MgCl2 is optimal for a satisfactory yield. In some cases, MgCl2 titration is needed for a better yield and specificity.
Application
Endpoint PCR;
qPCR;
RT-PCR;
RT-qPCR
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