Ready to load PCR Master Mix for routine applications.
*Load directly to gel after PCR
*Suitable for templates up to 5 kb
*Reaction set-up and shipment without dry ice
*All-in-one master mix format reduces pipetting errors and saves time
This master mix contains thermostable Taq polymerase FIREPol®, MgCl2, dNTPs, and buffer with detergent. You just need to add a template, primers, and water.
Ready to Load format also includes a compound needed for direct loading onto an agarose gel and two tracking dyes (blue and yellow) that allow monitoring progress during electrophoresis.
We recommend using FIREPol® Master Mix Ready to Load in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining. FIREPol® Master Mix Ready to Load is not recommended for use in post-PCR applications where spectrophotometric measurements (absorbance or fluorescence) are necessary because yellow and blue dyes can interfere with these applications.