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HOT FIREPol® DNA Polymerase Kit

HOT FIREPol® DNA Polymerase Kit
For research use only.

Some applications of this product  may require a license which is not provided by the purchase of this product.

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Description
Hot-start DNA Polymerase with a unique 30-day stability at room temperature for your everyday PCR needs.
A chemically modified hot-start version of the thermostable Taq DNA polymerase FIREPol®. This enzyme is activated only by pre-incubation at 95°C, preventing any unspecific polymerase activity at lower temperatures during reaction set-up.

*Increased specificity and sensitivity
*Reduced primer-dimer formation
*Reaction set-up and shipment without ice

HOT FIREPol® is inactive at room temperature and is activated by a 15 min incubation step at 95°C. This enables hot-start PCR and minimizes mispriming and extension from non-specifically annealed primers and primer-dimers. Recommended for routine applications (fragment up to 5 kb).

Possesses 5′→3′ polymerase and 5′→3′ exonuclease activity, as well as a non-template-dependent terminal transferase activity, but lacks a 3′→5′ exonuclease (proofreading) activity making the generated product suitable for TA-cloning.

The fidelity of HOT FIREPol® is similar to a regular Taq DNA Polymerase (error rate per nucleotide app. 2.5 x10-5).
Application
PCR;
qPCR;
Pre-amplification for Sanger sequencing; Pre-amplification for NGS;
Genotyping;
Colony PCR;
TA cloning
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