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HOT FIREPol® EvaGreen® HRM Mix (ROX)

HOT FIREPol® EvaGreen® HRM Mix (ROX)
For research use only.

Some applications of this product  may require a license which is not provided by the purchase of this product.

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Description
Optimized ready-to-use solution for High Resolution Melt (HRM) Analysis
* Highly sensitive mix for accurate SNP detection
* Sensitive EvaGreen® dye for reliable HRM performance
* Reaction set-up without dry ice

High-Resolution Melt (HRM) analysis is a fast and cost-effective method for post-PCR analysis to detect single nucleotide polymorphisms (SNP genotyping), genetic mutations, and DNA sequence variations.

HOT FIREPol® EvaGreen® HRM Mix is an optimized ready-to-use solution for HRM analysis, incorporating EvaGreen® dye.

It comprises all the components necessary to perform qPCR and HRM Analysis: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2, EvaGreen® dye, and ROX dye according to system requirements. The user simply needs to add water, template, and primers.

HOT FIREPol® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers.
Application
High-Resolution Melt (HRM);
SNP detection;
dye-based qPCR
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