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HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX)

HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX)
For research use only.

Some applications of this product  may require a license which is not provided by the purchase of this product.

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Description
Cost-effective dye-based qPCR Mix with passive reference dye with ROX.

* High sensitivity and specificity
* Excellent efficiency
* Reaction set-up and shipment without dry ice
* Cost-effective solution for a wide range of applications

Dye-based real-time quantitative PCR (qPCR) uses DNA binding dye to evaluate the DNA amplification process during PCR. In this mix EvaGreen® double-stranded DNA binding dye is used instead of the more widely used SYBR Green I that has similar fluorescence spectra. Compared to SYBR Green I dye EvaGreen® dye shows a higher fluorescence level, high sensitivity for detecting low template concentrations, and high stability at room temperature.

HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX) is an optimized ready-to-use solution for dye-based real-time quantitative PCR assays on cyclers that require passive reference dye (including high ROX or low ROX reference signal requiring platforms).
Application
Detection and quantification of DNA and cDNA targets;
Profiling gene expression;
Microbial detection;
Viral load determination
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