HOT FIREPol® Multiplex qPCR Mix (no ROX)
For research use only.
Some applications of this product may require a license which is not provided by the purchase of this product.
Ordering
- Catalog number: 08-01-00001-10
- Product Size: 10ml (10-pack: 10 x 1 ml; 2500 rxn)
- Product datasheet
- Product flyer
Description
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction.
Probe-based qPCR master mix that has been optimized for highly sensitive and accurate quantification of up to 4 targets in a single reaction. This master mix was developed for TaqMan® probes but is also suitable for other hydrolysis probe types
* Analyze 1-4 targets in 1 reaction
* High specificity and sensitivity
* Robust amplification of GC-rich targets
* Contains dUTP to prevent cross-contamination when used in combination with UNG
HOT FIREPol® Multiplex qPCR Mix is optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays. The qPCR Mix comprises all the components necessary (except primers, probes, and template) to perform qPCR: HOT FIREPol® DNA Polymerase, optimized buffer components, ultrapure dNTPs, and MgCl2.
HOT FIREPol® Multiplex qPCR Mix is optimized for DNA hydrolysis probes based on the 5′ flap endonuclease activity.
HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
Probe-based qPCR master mix that has been optimized for highly sensitive and accurate quantification of up to 4 targets in a single reaction. This master mix was developed for TaqMan® probes but is also suitable for other hydrolysis probe types
* Analyze 1-4 targets in 1 reaction
* High specificity and sensitivity
* Robust amplification of GC-rich targets
* Contains dUTP to prevent cross-contamination when used in combination with UNG
HOT FIREPol® Multiplex qPCR Mix is optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays. The qPCR Mix comprises all the components necessary (except primers, probes, and template) to perform qPCR: HOT FIREPol® DNA Polymerase, optimized buffer components, ultrapure dNTPs, and MgCl2.
HOT FIREPol® Multiplex qPCR Mix is optimized for DNA hydrolysis probes based on the 5′ flap endonuclease activity.
HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
Application
Detection and quantification of DNA and cDNA targets;
Profiling gene expression;
Microbial detection;
Viral load determination
Profiling gene expression;
Microbial detection;
Viral load determination
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