Sensitive qPCR Mix designed for effective quantification of DNA targets with probe-based chemistry.
Cost-effective real-time qPCR master mix for probe-based qPCR assays.
This master mix has been developed for TaqMan® probes but is suitable for other hydrolysis probe types as well.

* Suitable for singleplex and duplex assays
* High specificity and sensitivity
* Reaction set-up and shipment without dry ice

HOT FIREPol® Probe qPCR Mix Plus (Capillary) is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe.

The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. HOT FIREPol® Probe qPCR Mix Plus (Capillary) is optimized for DNA hydrolysis probes based on the 5´ flap endonuclease activity.

HOT FIREPol® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.