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Home » PCR qPCR and RT-qPCR reagents » qPCR and RT-qPCR » Dye-based qPCR Master Mixes » HOT FIREPol® SolisGreen® qPCR Mix 2.0

HOT FIREPol® SolisGreen® qPCR Mix 2.0

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For research use only.

Some applications of this product  may require a license which is not provided by the purchase of this product.

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Description
HOT FIREPol® SolisGreen® qPCR Mix 2.0 is a 5x-concentrated solution specially designed for dye-based real-time quantitative PCR (qPCR) assays. SolisGreen® dsDNA binding dye is incorporated into the product to evaluate the DNA amplification process during PCR. The fluorescence spectra of this dye are similar to the more widely used SYBR® Green I and is compatible with most major real-time cyclers. When compared to SYBR® Green I, SolisGreen® dye shows: higher fluorescence level, better sensitivity for detecting low template concentrations, great stability for room temperature storage.

HOT FIREPol® SolisGreen® qPCR Mix 2.0 is an optimized ready-to-use solution that contains all the necessary components, except sample (DNA template) and primers, to perform reactions with accurate and highly sensitive results. Additionally, the product includes a passive reference based on ROX dye making it compatible with both ROX-dependent and ROX-independent qPCR cyclers.

The product contains HOT FIREPol® DNA Polymerase to prevent the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
Application
Gene expression analysis and absolute quantification.
Pathogen detection and quantification.
Low-copy gene detection.
Cell-free DNA analysis.
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